Automated dispensing without temperature control
Animal derived materials used for 3D cell culture typically go through the polymerization of gels, which is caused by temperature. For scaling up assays in HTS this requires extensive temperature control and cooling down of instruments and reagents, so that the gels do not polymerize during dispensing. During screening, unexpected polymerization can clog pipette tips, cause variation in dispensing amount and lead to higher consumption of reagents, which increases the total cost of the assay. With GrowDex hydrogels you can do automated dispensing accurately and fast in room temperature.
Imaging and detection methods
The detection of fluorescent signals, for cell viability and proliferation assays is of key importance. Animal derived culture matrices often interfere with measurement caused by autofluorescence coming from the animal proteins. GrowDex hydrogels are not autofluorescent and contain only ultrapure water and nanocellulose.
Drug diffusion in compound screening
The diffusion of small and large molecules is crucial for compound screening. As the animal-based materials contain ECM proteins, the matrix can interfere with the diffusion of molecules. Especially large molecules, such as antibodies and ECM binding molecules can cause challenges for studying the effects of the drug compound. In GrowDex hydrogels small and large molecules are able to diffuse through the material, as it mainly contains water and only a small amount of nanocellulose fibers.
To detect and recover RNA and DNA for downstream processing the animal-based hydrogels interfere with antibody labeling, purification and detection. Using our GrowDase cellulase enzyme, you can recover your precious cells from GrowDex hydrogels, without affecting the cells. GrowDase cellulase enzyme degrades only cellulose fibers and it does not affect the cell proteins, structure or viability.