UPMBiomedicals

3D cell culture - meet the experts

Welcome to meet our experts in our new 3D cell culture webinar series. Here you can register to the live webinars and watch the recorded sessions later on. Stay up-to-date on our upcoming webinars.

 
Hanna Vuorenpää
Hanna Vuorenpää is working as a Postdoctoral Research Fellow in Adult Stem Cell Group in the Faculty of Medicine and Health Technology, Tampere University. She is responsible for establishment of functional vasculature in different Organ-on-Chip models. Currently, she focuses in development of vascularized white adipose tissue model to study obesity and its related disorders in Prof. Susanna Miettinen´s group. In 2016, Hanna Vuorenpää received her PhD in the Finnish Centre for Alternative Methods (currently FHAIVE) in Tampere University. She is experienced in development and validation of in vitro models in replacing animal experiments.

Part 10. Organ-on-Chip Technology

Tuesday, 31st May 2022, 15:30 to 16:00 CET

Sign up for the webinar

Presenter: Hanna Vuorenpää, Adult Stem Cell Group, Tampere University, Finland

The following topics will be discussed in the webinar:

  • GrowDex in Organ-on-Chips
  • Organ-on-Chip technology in tissue engineering
  • Functional vascularization in tissue models

After the webinar there is a Q&A session (15 minutes)

 
Beate Rinner is Assoc-Prof at the Medical University of Graz, Head of the Core Facility Alternative Biomodels and Preclincial Imaging and founder of the Cellbank Graz. Her research focuses on the establishment of rare tumor cell lines and the generation of autologous tumor models to advance personalized medicine and to identify and understand important signaling pathways. Additionally, cell communication and biomarker development through the release of extracellular vesicles are among her areas of expertise.

Part 9. Human Biopsies in Nanofibrillar Cellulose Hydrogel (GrowDex) – A Novel Method for Long-term Tissue Culture

Tuesday, 5th April 2022, 15:30 to 16:00 CET

WATCH THE RECORDING!

Presenter: Beate Rinner, Medical University of Graz

Advanced 3D in vitro models are laborious to prepare and susceptible to unintentional design errors due to culture adaptations, cell immaturity, xenofactors or yet incomplete knowledge of the dynamics within tissues or materials. In order to acquire cost-efficient research material with intact in vivo composition, we developed novel tissue culture method with plant-derived scaffolding.

Human skin-, foreskin- and glioblastoma multiforme biopsies were dissected mechanically and cultivated for 28 days in plant-derived nanofibrillar cellulose hydrogel. Comparative cultures were done using mouse sarcoma tumor –derived Matrigel™. Long-term preservation of cultivated tissues was evaluated against typical immunohistochemical biomarkers for each tissue type: skin tissues for cytokeratins 5/6, E-cadherin and vimentin for sustained tissue structures, and brain neoplasia for Olig2, S100, Nestin, NOTCH1, MAP2 and GFAP for preserved disease profile.

Histological analysis from both culture conditions showed that until day 28, all cultivated biopsy types were able to sustain their characteristic protein expressions without signs of necrosis. We here conclude a novel tissue culture model in xeno-free 3D scaffolding, that can enable long-term sample storage in vitro, studies of human tumor tissues and their non-neoplastic microenvironment, and innovations in personalized medicine research.

After the webinar there is a Q&A session (15 minutes)

 
Joe Trask, PerkinElmer
Joe Trask is the North America Product Line Leader in the Cellular Imaging and Analysis group at PerkinElmer. He is instrumental in strategic customer support through teaching, training, and collaborations. He brings over 25 years of experience in high content screening technology from academia, pharmaceutical, and biotechnology studying cancer, immunology, neurodegeneration, and toxicology. Joe has published several research articles and book chapters in High Content Imaging field. Joe is an associated editor for the NIH/NCATS Assay Guidance Manual and was a co-founder and first President of Society for Biomolecular Imaging and Informatics (SBI2).

Part 8. HCS Imaging Strategies to Interrogate 3D Cell Models

Tuesday, 16th November 2021, 15:00 to 15:30 CET

WATCH THE RECORDING!

Presenter: Joe Trask, PerkinElmer

HCS Imaging Strategies to Interrogate 3D Cell Models

  • Today’s 3D HCS imaging workflow
  • Intelligent image acquisition to save time and data space
  • Advances to measure inside the 3D cell model

After the webinar there is a Q&A session (15 minutes)

 
Tijmen Booij
About Presenter: Tijmen studied Bio-Pharmaceutical Sciences at Leiden University in the Netherlands and performed his PhD degree on the topic of phenotypic screening of 3D cell cultures, with a focus on Polycystic Kidney Disease, in the Leiden Academic Centre for Drug Research (LACDR). During his PhD studies, Tijmen learned to work with various 3D-cell culturing techniques, phenotypic screening, and lab automation. After completing his PhD studies in 2017, Tijmen moved to Zürich to work as screening specialist for the NEXUS theragnostics discovery unit, a technology platform of ETH Zürich. Here Tijmen is currently responsible for assay development, with a focus on advanced cell-culture models, and for maintaining the in-house compound collection, and has over the past three years developed fully automated 3D-cell culture and organoid high-throughput screens at NEXUS.

Part 7. Automating liver cancer organoid drug screens

Tuesday, 8th June 2021, 15:30 to 16:00 CET

WATCH THE RECORDING!

Presenter: Dr. Tijmen Booij, Screening Specialist, NEXUS Personalized Health Technologies

Over the last years, drug screens with organoids have gained in popularity due to their improved physiological relevance, which is expected to reduce the number and size of in vivo studies that are required to confirm in vitro results.

Currently most organoid screens are being performed in the range of hundreds to perhaps a few thousand compounds per screen. From the technical perspective, setting up high-throughput screens with organoids is still a challenge. On the one hand this is due to the availability of sufficient organoids, but on the other hand also because of the reagents that are traditionally used.

As technology platform of the ETH Zürich, NEXUS has over the last couple of years performed several successful organoid screens for different disease systems, where we observed that animal-derived hydrogels are a consistent source of variation and additionally complicate the automation of screening assays.

To improve throughput and remove potential sources of variation in our organoid screens, we optimized screening conditions using organoid models for Hepatocellular Carcinoma with UPM Biomedicals’ GrowDex and GrowDex-T. We report the successful adaptation of our organoid screening methodology for these HCC organoid lines, and expect that a similar conversion will be possible for many other organoid types.

After the webinar there is a Q&A session (15 minutes)

 

Part 6. 3D high-content imaging, image analysis and phenomics

Tuesday, 13th April 2021, 15:30 to 16:00 CET

WATCH THE RECORDING!

Presenter: Antti Hassinen, Institute for Molecular Medicine Finland

  • Sample treatment of 3D cell cultures for high-content microscopy
  • 3D Image analysis of cells and organoids
  • Drug sensitivity and resistance testing with imaging readouts

After the webinar there is a Q&A session (15 minutes)

About Presenter:

Antti Hassinen is the head of FIMM-HCA unit. He has previously worked in the Faculty of Biochemistry and Molecular Medicine in University of Oulu and gained his PhD in the group of Adj Prof Sakari Kellokumpu in 2013 working in the field of glycobiology and Golgi homeostasis in cancer cells. In the work he gained experience in protein chemistry, molecular biology, cell biology, cytometry and microscopy techniques as well as high throughput imaging. His work in FIMM-HCA is focused on providing open access high-throughput microscopy services to academic and commercial customers and collaborators. One of the main research focuses is the development of 3D high-throughput microscopy for use in drug sensitivity and resistance testing in close collaboration with the HTB FIMM unit.

Read more about HTS/HCS with GrowDex

 

Part 5. High content imaging in patient-derived models

Tuesday, 9th February 2021, 15:30 to 16:00 CET

WATCH THE RECORDING!

Presenter: Brinton Seashore-Ludlow, SciLifeLab and Karolinska Institutet

  • 3D models in high content imaging
  • Primary models in high throughput screening
  • High dimensional data for drug repositioning

After the webinar there is a Q&A session (15 minutes)

About Presenter:

Brinton Seashore-Ludlow received her PhD from KTH. She then did a Postdoc in the lab of Stuart Schreiber at the Broad Institute of Harvard and MIT. Her work there focused on elucidating predictors of drug response in a large-scale cell line profiling dataset. Brinton then moved to the Chemical Biology Consortium Sweden at SciLifeLab. Currently Brinton is an assistant professor in the team headed by Olli Kallioniemi at SciLifeLab. Her work focuses on high content imaging in primary cells.

Read more about HTS/HCS with GrowDex

 

Part 4. Human neuronal cells and 3D culturing

Wednesday, 16th December 2020, 15:30 to 16:00 CET

WATCH THE RECORDING

Presenter: Susanna Narkilahti, Adj. Prof, NeuroGroup, Faculty of Medicine and Health Technology, Tampere

  • 3D neuronal cell cultures, relevance compared to brain organoids
  • Challenges of 3D neuronal cultures
  • Future aspects towards multi-organ cultures

After the webinar there is a Q&A session (15 minutes)

About Presenter:

Susanna Narkilahti received Master degree in biochemistry from University of Oulu in 2000 and PhD in Neurobiology from University of Kuopio in 2005 and Adjunct Professor status in Stem cells and Tissue Engineering from University of Tampere in 2010. She initiated the NeuroGroup in 2006 and has thereafter been group leader. SN has extensive and internationally acknowledged knowhow in neurologic field including epilepsy research and pluripotent stem cell applications. She has participated several national and international projects and currently is leader of Academy of Finland Modular platform for epilepsy modeling in vitro (MEMO) consortium project and PI in Academy of Finland, Centre of Excellence in Body-On-Chip Research. In Faculty of Medicine and Health Technology, she is a leader of two core facilities; BMT Imaging Core and BMT Electrophysiology Core. The research of SN has focused on human pluripotent stem cells and in vitro modeling and her main interest/activities are: production and characterization of human pluripotent stem cell derived neural cells, controlled/guided 2D and 3D cultures, disease modeling, in vitro modeling, functionality of neuronal networks in vitro, and data analysis.

Read more about Neural applications with GrowDex

 

Part 3. Liver is not flat – why your liver model should be?

Tuesday, 20th October 2020, 15:30 to 16:00 CET

WATCH THE RECORDING

Application Scientist Lauri Paasonen will guide you through:

  • Produce liver organoids with full control of the environment
  • Reproducible and HTS capable toxicity screens
  • Easy analysis and more physiologically relevant biological response also in long term assays

After the webinar there is a Q&A session (15 minutes)

About Presenter: Lauri Paasonen has a Ph.D in pharmaceutical sciences and is an Application Scientist at UPM Biomedicals, focusing on 3D cell culture development.

 

Part 2. A blank canvas for your stem cell culture.

Tuesday, 25th August, 2020, 15:30 to 16:00 CET

WATCH THE RECORDING

Application Scientist Jonathan Sheard will guide you through:

  • What does animal free mean for stem cell cultures

  • Where did it all start for stem cells in GrowDex

  • New and novel applications of stem cells in GrowDex (15 minutes)

After the webinar there is a Q&A session (15 minutes)

About Presenter: Jonathan Sheard has a Ph.D. in biomedical sciences and is an Application Scientist at UPM Biomedicals, focusing on Stem Cells and 3D cell culture development.

Part 1. Setting up reproducible and scalable assays

Tuesday, 23rd June, 2020, 15:30 to 16:00 CET

WATCH THE RECORDING

Application Scientist Lauri Paasonen will guide you quickly through:

  • What, Why and How of 3D cell culture
  • Advantages of using animal-free, well-defined hydrogel
  • Introduction of GrowDex 3D cell culture applications
    (15 minutes)

After the webinar there is a Q&A session (15 minutes)

About Presenter: Lauri Paasonen has a Ph.D in pharmaceutical sciences and is an Application Scientist at UPM Biomedicals, focusing on 3D cell culture development.