A Portable, Negative-Pressure Actuated, Dynamically Tunable Microfluidic Droplet Generator

Trossbach, M., M. de Lucas Sanz, B. Seashore-Ludlow and H. N. Joensson (2022)

Abstract

"Droplet microfluidics utilize a monodisperse water-in-oil emulsion, with an expanding toolbox offering a wide variety of operations on a range of droplet sizes at high throughput. However, translation of these capabilities into applications for non-expert laboratories to fully harness the inherent potential of microscale manipulations is woefully trailing behind. One major obstacle is that droplet microfluidic setups often rely on custom fabricated devices, costly liquid actuators, and are not easily set up and operated by non-specialists. This impedes wider adoption of droplet technologies in, e.g., the life sciences. Here, we demonstrate an easy-to-use minimal droplet production setup with a small footprint, built exclusively from inexpensive commercially sourced parts, powered and controlled by a laptop. We characterize the components of the system and demonstrate production of droplets ranging in volume from 3 to 21 nL in a single microfluidic device. Furthermore, we describe the dynamic tuning of droplet composition. Finally, we demonstrate the production of droplet-templated cell spheroids from primary cells, where the mobility and simplicity of the setup enables its use within a biosafety cabinet. Taken together, we believe this minimal droplet setup is ideal to drive broad adoption of droplet microfluidics technology."

 

Read publication

 

Trossbach, M., M. de Lucas Sanz, B. Seashore-Ludlow and H. N. Joensson (2022). "A Portable, Negative-Pressure Actuated, Dynamically Tunable Microfluidic Droplet Generator." Micromachines (Basel) 13(11).

Nanofibrillated Cellulose for Skin Substitutes
Story | 2 min

Nanofibrillated Cellulose for Skin Substitutes

Read more
How biomimetic nanofibers advance the realm of cutaneous wound management: The state-of-the-art and future prospects
Story | 2 min

How biomimetic nanofibers advance the realm of cutaneous wound management: The state-of-the-art and future prospects

Read more
Fantastic Event Through Collaboration!
Story | 2 min

Fantastic Event Through Collaboration!

Read more