Silica bioreplication preserves three-dimensional spheroid structures of human pluripotent stem cells and HepG2 cells

Lou Y.-R, et al. 2015

Abstract

"Three-dimensional (3D) cell cultures produce more in vivo-like multicellular structures such as spheroids that cannot be obtained in two-dimensional (2D) cell cultures. Thus, they are increasingly employed as models for cancer and drug research, as well as tissue engineering. It has proven challenging to stabilize spheroid architectures for detailed morphological examination. Here we overcome this issue using a silica bioreplication (SBR) process employed on spheroids formed from human pluripotent stem cells (hPSCs) and hepatocellular carcinoma HepG2 cells cultured in the nanofibrillar cellulose (NFC) hydrogel. The cells in the spheroids are more round and tightly interacting with each other than those in 2D cultures and they develop microvilli-like structures on the cell membranes as seen in 2D cultures. Furthermore, SBR preserves extracellular matrix-like materials and cellular proteins. These findings provide the first evidence of intact hPSC spheroid architectures and similar fine structures to 2D-cultured cells, providing a pathway to enable our understanding of morphogenesis in 3D cultures."

 

Read publication

 

Lou Y.-R, et al. Silica bioreplication preserves three-dimensional spheroid structures of human pluripotent stem cells and HepG2 cells. Scientific Reports 2015; 5, 13635.

An animal-free hydrogel for translational studies in vitro - in vivo - in human
Story | 1 min

An animal-free hydrogel for translational studies in vitro - in vivo - in human

Read more
Multiplex Analysis of 3D Liver Cell Cultures in GrowDex®
Story | 14 min

Multiplex Analysis of 3D Liver Cell Cultures in GrowDex®

Read more
Three-dimensional cell culture of human mesenchymal stem cells in nanofibrillar cellulose hydrogels
Story

Three-dimensional cell culture of human mesenchymal stem cells in nanofibrillar cellulose hydrogels

Read more