Three-Dimensional Otic Neuronal Progenitor Spheroids Derived from Human Embryonic Stem Cells

Heuer, R. A., K. T. Nella, H.-T. Chang, K. Coots, A. Oleksijew, C. Roque, L. H. A. Silva, T. McGuire, K. Homma and A. J. Matsuoka (2020)

Abstract

"Stem cell–replacement therapies have been proposed as a potential tool to treat sensorineural hearing loss by aiding the regeneration of spiral ganglion neurons (SGNs) in the inner ear. However, transplantation procedures have yet to be explored thoroughly to ensure proper cell differentiation and optimal transplant procedures. We hypothesized that the aggregation of human embryonic stem cell (hESC)–derived otic neuronal progenitor (ONP) cells into a multicellular form would improve their function and their survival in vivo post-transplantation. We generated hESC–derived ONP spheroids—an aggregate form conducive to differentiation, transplantation, and prolonged cell survival—to optimize conditions for their transplantation. Our findings indicate that these cell spheroids maintain the molecular and functional characteristics similar to those of ONP cells, which are upstream in the SGN lineage. Moreover, our phenotypical, electrophysiological, and mechanical data suggest an optimal spheroid transplantation point after 7 days of in vitro three-dimensional (3D) culture. We have also developed a feasible transplantation protocol for these spheroids using a micropipette aided by a digital microinjection system. In summary, the present work demonstrates that the transplantation of ONP cells in spheroid form into the inner ear through micropipette 7 days after seeding for 3D spheroid culture is an expedient and viable method for stem cell replacement therapies in the inner ear."

 

Read publication

 

Heuer, R. A., K. T. Nella, H.-T. Chang, K. Coots, A. Oleksijew, C. Roque, L. H. A. Silva, T. McGuire, K. Homma and A. J. Matsuoka (2020). "Three-Dimensional Otic Neuronal Progenitor Spheroids Derived from Human Embryonic Stem Cells." Tissue Engineering Part A.

An animal-free hydrogel for translational studies in vitro - in vivo - in human
Story | 1 min

An animal-free hydrogel for translational studies in vitro - in vivo - in human

Read more
Multiplex Analysis of 3D Liver Cell Cultures in GrowDex®
Story | 14 min

Multiplex Analysis of 3D Liver Cell Cultures in GrowDex®

Read more
Surface modifications of nanocellulose for drug delivery applications; a critical review
Story | 1 min

Surface modifications of nanocellulose for drug delivery applications; a critical review

Read more