Engineering a Three Dimensional Stem Cell Niche in the Inner Ear by Applying a Nanofibrillar Cellulose Hydrogel with a Sustained Release Neurotrophic Factor Delivery System

Hsiang Tsun Chang, Rachel A. Heuer , Andrew M. Oleksijew, Kyle S. Coots, Christian B. Roque, Kevin T. Nella, Tammy L. McGuire and Akihiro J. Matsuoka

Background

The application of human embryonic stem cells (hESCs) in stem cell replacement therapies in the inner ear is hindered by low cell survival post-transplant. Here, we aim to enhance the in vitro and in vivo survival rate and neuronal differentiation of otic neuronal progenitors (ONPs) by generating an artificial stem cell niche consisting of three dimensional (3D) hESC derived ONP spheroids, a nanofibrillar cellulose hydrogel, and a sustained release brain derivative neurotrophic factor (BDNF) delivery system.
 
 
 
Feasibility study for the long-term storage of human cells using novel hydrogels
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Feasibility study for the long-term storage of human cells using novel hydrogels

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Otic Neuronal Differentiation of Three Dimensional Spheroids Derived from Human Pluripotent Stem Cells: Characterization Towards In Vivo Transplantation
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Otic Neuronal Differentiation of Three Dimensional Spheroids Derived from Human Pluripotent Stem Cells: Characterization Towards In Vivo Transplantation

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Cost-effective Techniques for 3D Culture of Heterotypic Tumor Models
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Cost-effective Techniques for 3D Culture of Heterotypic Tumor Models

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