UPMBiomedicals
  • Resource Center
  • Publications
  • Engineering a Three Dimensional Stem Cell Niche in the Inner Ear by Applying a Nanofibrillar Cellulose Hydrogel with a Sustained Release Neurotrophic Factor Delivery System

Engineering a Three Dimensional Stem Cell Niche in the Inner Ear by Applying a Nanofibrillar Cellulose Hydrogel with a Sustained Release Neurotrophic Factor Delivery System

Hsiang Tsun Chang, Rachel A. Heuer , Andrew M. Oleksijew, Kyle S. Coots, Christian B. Roque, Kevin T. Nella, Tammy L. McGuire and Akihiro J. Matsuoka

Background

The application of human embryonic stem cells (hESCs) in stem cell replacement therapies in the inner ear is hindered by low cell survival post-transplant. Here, we aim to enhance the in vitro and in vivo survival rate and neuronal differentiation of otic neuronal progenitors (ONPs) by generating an artificial stem cell niche consisting of three dimensional (3D) hESC derived ONP spheroids, a nanofibrillar cellulose hydrogel, and a sustained release brain derivative neurotrophic factor (BDNF) delivery system.
 
 
 
Long-term Culture of Human Biopsies in Nanofibrillar Cellulose Hydrogel
Story | 11 min

Long-term Culture of Human Biopsies in Nanofibrillar Cellulose Hydrogel

Read more
Scalable and Automated Three-Dimensional Cell Cultures for High-Throughput and High-Content Screening
Story

Scalable and Automated Three-Dimensional Cell Cultures for High-Throughput and High-Content Screening

Read more
RNA isolation and integrity from primary tumor PDECs cultured in GrowDex compared with Cultrex
Story | 10 min

RNA isolation and integrity from primary tumor PDECs cultured in GrowDex compared with Cultrex

Read more