Animal-free GrowDex® hydrogels are a proven solution for high throughput (HTS) and high content screening (HCS) in 3D cell culture assays
Key benefits of GrowDex® for HTS/HCS assays:
- More morphologically and functionally relevant cell models with 3D assays
- Automated dispensing without temperature control
- No autofluorescent signals in the matrix
- No ECM protein matrix interference
- One-step enzymatic rell recovery
- Scale your assays up with confidence; proven repeatability up-to 1536 well-format
Download guide: 5 Ways to improve your cell assays
Learn more about the 5 ways to improve your cell assays:
- Empower your cell-based research with 3D cell culture
- Create human-like 3D cell models with scaffold-based matrices
- Go animal-free
- Automate your liquid handling for unparalleled reproducibility
- Scale-up with confidence
Solve the common issues faced with animal derived materials in HTS
When trying to scale up animal derived matrix based -assays in HTS, extensive temperature control is required in automation, including cooling down of instruments and reagents, to prevent the gel polymerization during dispensing. During molecule screening, unexpected polymerization can clog pipette tips, cause variation in dispensing amount and lead to higher consumption of reagents. This increases the total cost of the assay. All added variation can also cause misleading biological responses and failure in target identification. With GrowDex hydrogels you can do automated dispensing accurately and fast in room temperature.
The detection of fluorescence signals, for cell viability and proliferation is of key for fluorescence-based HTS assays. Animal derived matrices often interfere with fluorescence measurement caused by autofluorescence coming from the animal proteins. GrowDex does not interfere with the readout as GrowDex hydrogels are not auto-fluorescent and contain only ultrapure water and nanocellulose.
In order to screen large compound libraries quickly and efficiently, large and small molecules are required to diffuse through cell culture matrix. As the animal-based materials contain ECM proteins and other biological components, these matrices can interfere with the diffusion of molecules, such as antibodies and molecules binding to ECM, causing challenges to study the drug compound effect. In GrowDex hydrogels, small and large molecules are able to diffuse through the material, as it mainly contains water and only a small amount of nanocellulose fibers.
Typically RNA and DNA are collected from living cells for downstream gene or protein expression studies. The animal-based hydrogels may interfere with purification and readouts as they contain animal DNA and RNA. Using our GrowDase cellulase, you can recover cells from GrowDex hydrogels, for downstream processes, such as flow cytometry. GrowDase cellulase enzyme degrades only cellulose fibers and it does not affect the cell surface proteins, structure or cell viability.
Read more about how you can improve your high throughput assays. Or contact us for more information.
HTS & HCS Videos
See our webinars and other video resources on cell culture assay automation, high throughput screening, and high content imaging.
High throughput screening and high content screening in drug discovery
In drug development, HTS and HCS enable hit-to-lead identification and setting up dose-response curves, by utilizing automation robotics, image-based data analysis and liquid handling devices that together help quickly to screen large chemical compound libraries. These tests are typically conducted on 96-, 384- or 1536-well microplates.
HTS/HCS assays generate large data sets that help to identify the right compounds to modulate a particular biomolecular pathway. High-quality data sets provide the starting point for the target identification in the drug discovery process. That is why, it is important to choose the right high-quality cell-based assays for further assay development and biochemical compound testing.
GrowDex hydrogels' natural and animal-free features as well as the quality control manufacturing process supports reproducible assays without lot-to-lot variability. These make GrowDex hydrogels especially fitting for large-scale high throughput and high content screening assays in microtiter plates.
High Throughput Instruments
Are you already working with a high throughput instrument or already know what instrument you are going to be using? Check out these instrument specific practical protocols to get you started with your assay.
High throughput and high content screening applications
Co-Culturing of Primary Hepatocytes and Kupffer Cells in GrowDex and DAPI/Phalloidin Staining of the Cell Spheroids
With over 150 different assay protocols available GrowDex is our original hydrogel. Highly biocompatible GrowDex has been used widely for the culture of primary cells through to iPS and ES cells.
GrowDex-T brings superior imaging quality for phase contrast and brightfield imaging. This hydrogel is completely transparent making imaging and automated analysis easy.