Animal-free GrowDex® hydrogels are a proven solution for high throughput (HTS) and high content screening (HCS) in 3D cell culture assays


Key benefits of GrowDex® for HTS/HCS assays:

  • More morphologically and functionally relevant cell models with 3D assays
  • Automated dispensing without temperature control
  • No autofluorescent signals in the matrix
  • No ECM protein matrix interference
  • One-step enzymatic cell recovery
  • Scale your assays up with confidence; proven repeatability up-to 1536 well-format



Download guide: 5 Ways to improve your cell assays


Learn more about the 5 ways to improve your cell assays:

  1. Empower your cell-based research with 3D cell culture
  2. Create human-like 3D cell models with scaffold-based matrices
  3. Go animal-free
  4. Automate your liquid handling for unparalleled reproducibility
  5. Scale-up with confidence


5 Ways Guide

Solve the common issues faced with animal derived materials in HTS


Read more about how you can improve your high throughput assays. Or contact us for more information.

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HTS & HCS Videos

See our webinars and other video resources on cell culture assay automation, high throughput screening, and high content imaging.

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High throughput screening and high content screening in drug discovery

In drug development, HTS and HCS enable hit-to-lead identification in drug discovery and setting up dose-response curves, by utilizing automation robotics, image-based data analysis and liquid handling devices that together help quickly to screen large chemical compound libraries. These tests are typically conducted on 96-, 384- or 1536-well microplates.

HTS/HCS assays generate large data sets that help to identify the right compounds to modulate a particular biomolecular pathway. High-quality data sets provide the starting point for the target identification in the drug discovery process. That is why, it is important to choose the right high-quality cell-based assays for further assay development and biochemical compound testing.

GrowDex hydrogels' natural and animal-free features as well as the quality control manufacturing process supports reproducible assays without lot-to-lot variability. These make GrowDex hydrogels especially fitting for large-scale high throughput and high content screening assays in microtiter plates.

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High Throughput Instruments

Are you already working with a high throughput instrument or already know what instrument you are going to be using? Check out these instrument specific practical protocols to get you started with your assay.

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Related Products


What is high throughput screening?

High Throughput Screening (HTS) is a cell culture automation method used to rapidly analyze thousands of samples. High throughput screening focuses on generating quantitative data as results. Transitioning from manual work to automated high throughput assays helps expedite the analysis of cell culture assays. When preparing assays for HTS, researchers can work more precisely with smaller volumes and use different well plate sizes, including 96-well, 384-well, and even 1536-well plates.

What is high content screening?

High Content Screening (HCS) is a cell culture automation method used for acquiring high-resolution microscopy images of cells, spheroids, or organoids. It aids in the identification of various compounds, such as small molecules, within analyzed samples. In High Content Screening assays, samples are dyed with, for example fluorescent dyes, to support the identification of different compounds. High Content Screening assays result in high-resolution images of the analyzed cells, spheroids, or organoids. High content imaging (HCI) and high content analysis (HCA) are synonymous terms for High Content Screening.

What's the difference between high throughput screening and high content screening?

While HTS and HCS may sound similar and both are cell culture automation methods, they have distinct roles in the automation process. High throughput screening (HTS) is primarily focused on analyzing numerous individual samples to generate a large amount of quantitative data, whereas high content screening (HCS) is geared towards acquiring high-resolution images for in-depth analysis of individual samples.

High Throughput Screening and High Content Screening in Drug Discovery

Both high throughput screening and high content screening can be utilized in the drug discovery process. High throughput screening is commonly used in drug discovery for viability and toxicity assays, facilitating the rapid identification of lead compounds. On the other hand, High Content Screening in drug discovery can be employed, for instance, to analyze potential drug side-effects and provide a more precise analysis of the effects of a selected lead compound.